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Welcome to the web server for the
ab-initio 3D reconstruction of symmetric particles
from 2D projections.

[-] Process overview:
Pass your mouse over each step to get an explanation.
Q: What do you need to do/know at this step ?

Please, enter the 4 letters above here:

and give a valid e-mail address to receive the results (optional):

  1. Enter the pixel size (in Angstroms):
  2. Enter the approximate particle size (in pixels): (You can get an approximation of the particle size with GIMP or Photoshop)
  3. Browse your disk and choose a TEM micrograph (100Mb Max) containing symmetric particles. (Do you need an image to test ? [get it here])

File check:

[+] Authors:
Leandro F. Estrozi <>
Jorge Navaza <>
[+] Disclaimer:
The authors disclaim any responsibility for the use or consequences of the use of the supplied information in any application or circumstances. They do not warrant or assume any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed.
[+] Acknowledgments:
A big thanks to Fred Metoz and the "Equipe Info" of the IBS for helping me with the server.
[+] FAQ:
[+] References:
  1. NAVAZA, J. ; "On the three-dimensional reconstruction of icosahedral particles.", Journal of Structural Biology, v. 144, p.13-23, 2003.
  2. ESTROZI, L. F. ; NAVAZA, J. "Fast projection matching for Cryo-electron Microscopy Image Reconstruction", Journal of Structural Biology, 162, p.324-334, 2008.
  3. *** If this server has been useful to you, please cite this reference:

  4. ESTROZI, L. F. ; NAVAZA, J. "Ab initio high-resolution single-particle 3D reconstructions: the Symmetry-Adapted-Functions way", Journal of Structural Biology, 172, p.253-260, 2010. [PDF]
  6. GRIGORIEFF, N. "FREALIGN: high-resolution refinement of single particle structures", Journal of Structural Biology, 157, p.117–125, 2007.
  7. BAKER, T.S. ; CHENG, R.H. "A model-based approach for determining orientations of biological macromolecules imaged by cryoelectron microscopy", Journal of Structural Biololy, 116(1), p.120-130, 1996.
  8. CROWTHER, R.A., "Procedures for Three-Dimensional Reconstruction of Spherical Viruses by Fourier Synthesis from Electron Micrographs", Phylosophical Transactions of the Royal Society B, v. 261, no.837, p. 221-230, 1971. (doi: 10.1098/rstb.1971.0054). [PDF]
  9. ESTROZI, L. F. ; TRAPANI, S. ; NAVAZA, J. "SCA: symmetry-based center assignment of 2D projections of symmetric 3D objects", Journal of Structural Biology, v. 157, p. 339-347, 2007.
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