Pass your mouse over each step to get an explanation.
Q: What do you need to do/know at this step ?
A: Upload a nice micrograph and inform the pixel size (in Angstroms) and the aproximate particle size (in pixels).
A: Click at some particles to select them (boxing) and choose the type of symmetry.
A: Be patient and wait ~10 minutes for the miracle to happen :-) Suggestion: Read the paper about the method!
A: Check for the repeatability and consistency of the result. Start again by picking other particles and see if you get similar results. Inspect the correlation profiles and the 3D reconstructions based on single particles. Particles with a unique and well defined correlation peak are the best. Make sure that the automatically selected reference particle is a good one. You can also use the troubleshooting options (at the particle-picking page) if necessary.
Leandro F. Estrozi <leandro.estrozi
Jorge Navaza <jorge.navaza
The authors disclaim any responsibility for the use or consequences of the use of the supplied information in any application or circumstances. They do not warrant or assume any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed.
A big thanks to Fred Metoz and the "Equipe Info" of the IBS
for helping me with the server.
NAVAZA, J. ; "On the three-dimensional reconstruction of icosahedral particles.", Journal of Structural Biology, v. 144, p.13-23, 2003.
ESTROZI, L. F. ; NAVAZA, J. "Fast projection matching for Cryo-electron Microscopy Image Reconstruction", Journal of Structural Biology, 162, p.324-334, 2008.
*** If this server has been useful to you, please cite this reference:
ESTROZI, L. F. ; NAVAZA, J. "Ab initio high-resolution single-particle 3D reconstructions: the Symmetry-Adapted-Functions way", Journal of Structural Biology, 172, p.253-260, 2010. [PDF]
GRIGORIEFF, N. "FREALIGN: high-resolution refinement of single particle structures", Journal of Structural Biology, 157, p.117–125, 2007.
BAKER, T.S. ; CHENG, R.H. "A model-based approach for determining orientations of biological macromolecules imaged by cryoelectron microscopy", Journal of Structural Biololy, 116(1), p.120-130, 1996.
CROWTHER, R.A., "Procedures for Three-Dimensional Reconstruction of Spherical Viruses by Fourier Synthesis from Electron Micrographs", Phylosophical Transactions of the Royal Society B, v. 261, no.837, p. 221-230, 1971. (doi: 10.1098/rstb.1971.0054). [PDF]
ESTROZI, L. F. ; TRAPANI, S. ; NAVAZA, J. "SCA: symmetry-based center assignment of 2D projections of symmetric 3D objects", Journal of Structural Biology, v. 157, p. 339-347, 2007.